Journal: STAR Protocols

Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells

doi: 10.1016/j.xpro.2026.104519

Figure Lengend Snippet: Flow cytometric quality control of CD4 + T-cell sort via MACS technique

Article Snippet: CD4 + T-Cell isolation Kit, mouse , Miltenyi Biotec , Cat# 130-104-454.

Techniques: Control

Journal: STAR Protocols

Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells

doi: 10.1016/j.xpro.2026.104519

Figure Lengend Snippet: Gating strategy for further isolation of CD4 + T-cell populations via fluorescence-activated cell sorting

Article Snippet: CD4 + T-Cell isolation Kit, mouse , Miltenyi Biotec , Cat# 130-104-454.

Techniques: Isolation, Fluorescence, FACS

Journal: STAR Protocols

Article Title: Protocol for potent activation of T cells using BPI-stimulated murine bone marrow-derived cells

doi: 10.1016/j.xpro.2026.104519

Figure Lengend Snippet: Expected results of this protocol Naïve CD4 + T cells cultured for d5 in supernatant of untreated BMDCs (SN NT) or in supernatant of BPI-treated BMDCs (SN BPI). (A) Representative dot blot of flow cytometric analysis of CD62L and CD44 cell surface presentation. (B) IL-22 secretion measured by Luminex technology, n = 4. Data are shown as means ± SEM. Statistical testing was performed using Student`s ratio paired t test.

Article Snippet: CD4 + T-Cell isolation Kit, mouse , Miltenyi Biotec , Cat# 130-104-454.

Techniques: Cell Culture, Dot Blot, Luminex